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Femtomole peptide mapping by derivatization, high-performance liquid chromatography, and fluorescence detection

H Liu1, I S Krull, S A Cohen

  • 1Department of Chemistry, Northeastern University, 360 Huntington Avenue, Boston, Massachusetts 02115, USA.

Analytical Biochemistry
|June 20, 2001
PubMed
Summary

This study introduces a highly sensitive peptide mapping technique using derivatization and fluorescence detection, improving sensitivity 2000-fold. This advanced method enables comprehensive protein sequence coverage even with minimal sample amounts.

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