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Related Experiment Videos

Embryonal cell surface recognition. Extraction of an active plasma membrane component.

R Merrell, D I Gottlieb, L Glaser

    The Journal of Biological Chemistry
    |July 25, 1975
    PubMed
    Summary

    Proteins from chicken embryo neural membranes specifically inhibit cell aggregation. These solubilized proteins retain the same specificity as whole membranes, showing increased activity.

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    Area of Science:

    • Developmental biology
    • Neuroscience
    • Cell biology

    Background:

    • Plasma membranes from chicken embryo neural regions exhibit specific homotypic cell binding and aggregation inhibition.
    • Previous studies demonstrated this specificity in intact neural membranes.

    Purpose of the Study:

    • To solubilize and characterize proteins responsible for specific cell aggregation inhibition from neural plasma membranes.
    • To determine if the solubilized proteins retain the regional and temporal specificity of the original membranes.

    Main Methods:

    • Solubilization of proteins from neural retina and optic tectum plasma membranes using acetone delipidation and lithium diiodosalicylate extraction.
    • Assay of protein fractions for their ability to inhibit cell aggregation under standard conditions.

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    Main Results:

    • Proteins responsible for specific cell aggregation inhibition were successfully solubilized.
    • The extracted proteins demonstrated the same regional and temporal specificity as the original plasma membranes.
    • A highly purified protein fraction showed a 20-fold increase in specific activity compared to whole membranes, inhibiting aggregation of 2.5 x 10(-4) cells with just 2 micrograms.

    Conclusions:

    • The specific inhibition of neural cell aggregation is mediated by specific proteins within the plasma membrane.
    • These proteins can be isolated and retain their functional specificity, offering a more potent tool for studying cell recognition.