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Updated: Aug 6, 2026

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair
Published on: February 24, 2016
Human histology of new attachment after root coverage using subepithelial connective tissue graft
M Goldstein1, B D Boyan, D L Cochran
1Department of Periodontics, Hebrew University Hadassah Faculty of Dental Medicine, Jerusalem, Israel 91010.
Background, Aims:
Connective tissue grafts are used successfully in periodontal therapy for root coverage. However, reports on the histologic interface between the root surface and the grafted tissue have been few in number. This report describes a case study in which a subepithelial connective tissue graft was placed in a 27-year-old female on the maxillary left side.
Methods:
The graft (15 mm long, 10 mm wide, 1.5 mm thick) included palatal periosteum and was placed with the periosteal side facing the exposed bone and root surfaces.
Results:
15 weeks after grafting, the teeth presented with residual recessions of 1 mm, and buccal probing depths were approximately 1 mm. 14 months post-surgery, the 1st maxillary premolars on both sides were extracted for orthodontic therapy. Clinical parameters at the graft site remained as at 15 weeks. Histologic analysis of tooth #24 showed that the sulcular epithelium was keratinized; epithelium lining the dentin exhibited rete ridges projecting into the gingival connective tissue; and junctional epithelium extended over new cementum. New connective tissue attachment was also observed, including periodontal ligament.
Conclusion:
Biological width was comparable pre- and post-surgery, indicating a real gain in attachment of 3.9 mm.

