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Related Experiment Videos

The white blood cell differential: three methods compared.

R Siekmeier1, A Bierlich, W Jaross

  • 1Institut für Klinische Chemie und Laboratoriumsmedizin, Klinikum Carl Gustav Carus der Technischen Universität Dresden, Germany.

Clinical Chemistry and Laboratory Medicine
|July 4, 2001
PubMed
Summary
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Automated blood cell counters, like the ABX Pentra 120 Retic and Coulter STKS, show strong accuracy for white blood cell differentials compared to microscopy. These systems are reliable for routine haematological diagnostics.

Area of Science:

  • Hematology
  • Clinical Diagnostics
  • Laboratory Medicine

Background:

  • Automated blood cell counts are crucial for haematological diagnostics.
  • Microscopy is often the reference standard for white blood cell differential analysis, despite being labor-intensive.

Purpose of the Study:

  • To evaluate the ABX Pentra 120 Retic haematology analyser against the Coulter STKS system and microscopy.
  • To assess accuracy, precision, and reliability of automated analysers for white blood cell differentials.

Main Methods:

  • Comparison of 308 samples (adults and children, including oncology patients) using ABX Pentra 120 Retic, Coulter STKS, and microscopy.
  • Correlation analysis (Spearman's rank correlation coefficient, rs) for neutrophil, lymphocyte, monocyte, and eosinophil counts.

Related Experiment Videos

  • Evaluation of analytical performance for detecting morphological "left shift".

    • Main Results:

    • Strong correlations were observed between automated analysers and microscopy for neutrophils (rs ≥ 0.933), lymphocytes (rs ≥ 0.907), and eosinophils (rs ≥ 0.963).
    • Monocyte correlation showed moderate agreement (rs ≥ 0.459).
    • Sensitivity and specificity for detecting a "left shift" varied between analysers and depended on threshold settings.

    Conclusions:

    • The ABX Pentra 120 Retic and Coulter STKS haematology systems are suitable for routine haematological analysis.
    • Both automated systems demonstrate good performance in white blood cell differential counting.
    • Further optimization of "left shift" detection algorithms may be beneficial.