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Updated: Jul 30, 2026
Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
Published on: July 2, 2016
[Complete genome sequence analysis of the Hantavirus Z10 strain]
1National Institute for the Control of Pharmaceutical and Biological Pruducts, Beijing 100050, China.
Objective:
Study on the complete genome sequence of Hantavirus Z10 strain which has been applied for inactivated vaccine production in China, to assess its molecular characteristics and the diversity with other hantaviruses.
Methods:
The total RNA were prepared from Z10 virus infected cells and the RT-PCR products was cloned into T vector, sequenced and analyzed by using DNASTAR software.
Results:
The Z10 complete genome, L segment is 6,553, M segment is 3,615, S segment is 1,701 nucleotides in length, with a single open reading frame encoding 2,151, 1,135, 429 amino acids respectively. Sequence homology comparison showed that the 3 segment nucleotide of Z10 strain were close to HTN type virus, but only 83.6-87.4% homology with other HTN viruses at the nucleotide level. The phylogenetic analysis was made on their nucleotide and amino acid sequences.
Conclusion:
The results firstly demonstrates that Z10 strain is a new subtype of the Hantaan(HTN) type.
