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Micronuclei and other nuclear lesions as genotoxicity indicators in rainbow trout Oncorhynchus mykiss
1Departamento de Biologia Funcional, Area de Genetica, Facultad de Medicina, Universidad de Oviedo, c/Julian Claveria s/n, 33006 Oviedo, Spain. ayllon@sauron.quimica.uniovi.es
Abstract:
The induction of micronuclei and other nuclear abnormalities in renal erythrocytes of rainbow trout Oncorhynchus mykiss by six genotoxic compounds is evaluated. Colchicine, mitomycin, cyclophosphamide, acrylamide, methyl-methanesulfonate, and N-ethyl-N-nitrosourea were intraperitoneally injected in trout. Our results show that cyclophosphamide induces the formation of micronuclei and also the other nuclear abnormalities; N-ethyl-N-nitrosourea, acrylamide, and colchicine induce only micronuclei; mitomycin-C induces only other nuclear abnormalities but not micronuclei. Methyl-methanesulfonate does not induce nuclear abnormalities in rainbow trout at the dose assayed in this work. The possible genotoxic origin for the different nuclear abnormalities is discussed.
Insights
This study evaluated genotoxic compounds in rainbow trout. Cyclophosphamide induced both micronuclei and other nuclear abnormalities, while other compounds showed varied effects on fish genotoxicity.
Area of Science:
- Environmental Toxicology
- Aquatic Toxicology
- Genotoxicity Testing
Background:
- Rainbow trout (Oncorhynchus mykiss) are a model organism for aquatic toxicology.
- Evaluating genotoxic compounds is crucial for assessing environmental risks to aquatic ecosystems.
Purpose of the Study:
- To assess the genotoxic potential of six chemical compounds in rainbow trout.
- To determine the specific nuclear abnormalities induced by each compound in renal erythrocytes.
Main Methods:
- Intraperitoneal injection of rainbow trout with colchicine, mitomycin, cyclophosphamide, acrylamide, methyl-methanesulfonate, and N-ethyl-N-nitrosourea.
- Microscopic examination of renal erythrocytes for the induction of micronuclei and other nuclear abnormalities.
Main Results:
- Cyclophosphamide induced both micronuclei and other nuclear abnormalities.
- N-ethyl-N-nitrosourea, acrylamide, and colchicine induced only micronuclei.
- Mitomycin-C induced other nuclear abnormalities but not micronuclei.
- Methyl-methanesulfonate did not induce abnormalities at the tested dose.
Conclusions:
- Different genotoxic compounds exhibit varying patterns of nuclear abnormality induction in rainbow trout.
- The study highlights the differential sensitivity of fish erythrocytes to specific genotoxins.
- Further research is needed to elucidate the genotoxic mechanisms underlying observed abnormalities.
