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[Detection of Brucella melitensis by BACTEC 9050 system]
J Antonio Lepe1, F Javier Guerrero, A Garrido
1Sección de Microbiología. Hospital General de Riotinto. Huelva. jalepe@cica.es
Enfermedades Infecciosas Y Microbiologia Clinica
|July 7, 2001
Summary
The BACTEC 9050 system effectively detects Brucella spp. bacteremia, with 81.2% of patients identified. Extended incubation protocols are crucial for optimal Brucella detection.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Clinical Diagnostics
Background:
- Brucellosis is a significant zoonotic disease with potential for bacteremia.
- Early and accurate detection of Brucella bacteremia is critical for effective patient management.
- The BACTEC 9050 system is an automated blood culture system used for microbial detection.
Purpose of the Study:
- To assess the diagnostic capacity of the BACTEC 9050 system for detecting Brucella spp. bacteremia.
- To determine optimal incubation protocols for Brucella detection using the BACTEC 9050 system.
Main Methods:
- A study involving 16 febrile patients during a Brucella spp. epidemic.
- Blood cultures were processed using the BACTEC 9050 system with a 21-day incubation period.
- Serological tests (Rose Bengal, agglutination tube test) confirmed brucellosis.
Main Results:
- Brucella bacteremia was detected in 13 out of 16 patients (81.2%).
- The BACTEC 9050 system identified 11 cases, with detection times ranging from 3.4 to 10.2 days (mean 6.2 days).
- Two additional cases were identified via blind subculture after 21 days, indicating the need for extended incubation.
Conclusions:
- Standard 5-day incubation protocols are insufficient for the BACTEC 9050 system in suspected brucellosis cases.
- A 7-day incubation protocol would only detect approximately 69.2% of Brucella bacteremia cases.
- Extended incubation periods, up to 21 days with blind subculture, are recommended for maximizing Brucella detection with the BACTEC 9050 system.