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A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides
E Manzo1, A Molinaro, E Bedini
1Dipartimento di Chimica Organica e Biochimica, Università di Napoli 'Federico II', via Cintia, I-80126, Naples, Italy.
Abstract:
A novel mild procedure for the selective cleavage of ketosidic linkages is developed using ceric ammonium nitrate (CAN) in anhydrous N,N-dimethylformamide. Its application to lipopolysaccharides (LPS) is very significant because in the so far investigated LPS, the connection between the Lipid A region and the oligo(poly)saccharide part is always a keto-sugar. This procedure has been tested on LPS of Escherichia coli which contains Kdo as a linker between Lipid A and OPS and on Acinetobacter haemoliticus which contains D-glycero-D-talo-2-octulopyranosonic acid (Ko) as a linker and it performed efficiently in both cases.