Related Experiment Video
Updated: Jul 19, 2026

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
Published on: September 26, 2022
Combination therapy improves the recovery of the skin barrier function: an experimental model using a contact allergy
J P Hachem1, K De Paepe, E Vanpée
1Department of Dermatology, Vrije Universiteit Brussel, Belgium. jphachem@belcast.be
Background:
Nickel (Ni) allergic contact dermatitis (ACD) alters the skin barrier.
Objective:
Our aim was to compare the efficacy of combination therapies on ACD, using a topical corticosteroid and a corneotherapy agent (barrier cream), with that of a single therapy with corticosteroids.
Methods:
On day 1, 3 Ni test patches were applied on each forearm of 14 Ni-patch-test-positive females. Four contained 5% Ni and 2 physiological saline. Either topical corticosteroid or barrier cream were matched with the combination of both products on 3 of the 4 Ni ACD. The fourth was not treated. Clinical scoring, transepidermal water loss (TEWL) and stratum corneum (SC) capacitance were measured before (day 1) and after (days 4-8) ACD.
Results:
The combination therapy showed a significant decrease in TEWL values and an increase in SC capacitance.
Conclusion:
Combining a topical corticosteroid with corneotherapy agents prevents the delay in the healing process of skin barrier disruption due to ACD.
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