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Updated: Oct 5, 2026

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Published on: October 16, 2018
[Research of status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons]
1Department of Infectious Diseases, the First Clinic Hospital, Beijing Medical University, 100034 Beijing, China.
Objective:
This study was designed to investigate the different status of provirus HIV DNA in non-progressor and progressor of HIV-1 infected persons.
Methods:
HIV RNA, HIV DNA were detected with long-distance PCR (LD-PCR), reverse transcription PCR (RT-PCR), Southern blot and molecular hybridization from plasma and peripheral blood mononuclear cells (PBMCs), using primers locating between long terminal repeats LTR(U5)-LTR(R) and so on.
Results:
9.1 kb band is full-length HIV DNA genome which was present in all templates of 12 progressors. Deleted HIV DNA was observed in 9 of 12 progressors. Both the full-length and defective. provirus HIV DNA was detected in 5 of the 18 non-progressors. Defective HIV DNA was only detected in the other non-progressors.
Conclusions:
Clinical stages are closely related with both the full-length and the defective HIV DNA.
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