[Detection of Coxsackie B virus IgM antibody in myocarditis patients by Mac ELISA]
1Institute of Virology, Chinese Academy of Pre'oentire Medicine, Beijing 100052, China.
Insights
Coxsackie B virus IgM antibodies were detected in 69.6% of myocarditis patients, significantly higher than controls. Coxsackie B3 was the most prevalent type, indicating Cox B viruses are key causes of viral myocarditis.
Area of Science:
- Virology
- Immunology
- Cardiology
Background:
- Myocarditis is an inflammation of the heart muscle, often caused by viral infections.
- Coxsackie B viruses are known etiological agents of viral myocarditis.
Purpose of the Study:
- To detect Coxsackie B virus type 1-6 IgM antibodies in patients with clinically diagnosed myocarditis.
- To evaluate the prevalence of Coxsackie B virus IgM antibodies in myocarditis cases compared to a control group.
Main Methods:
- Capture IgM ELISA (Mac ELISA) was employed for antibody detection.
- 193 clinically diagnosed myocarditis cases and 181 non-myocarditis controls were analyzed.
Main Results:
- A total of 69.6% of myocarditis patients tested positive for Coxsackie B virus IgM antibodies.
- Coxsackie B3 was the most frequently detected type (40% of positive cases), followed by Cox B2, Cox B4, Cox B1, Cox B5, and Cox B6.
- The positive rate of Cox B virus IgM antibody in myocarditis patients was significantly higher than in the control group (P <0.01).
Conclusions:
- Coxsackie B viruses are likely the primary agents responsible for viral myocarditis in the Beijing population studied.
- Mac ELISA is an effective, rapid, specific, and sensitive method for early diagnosis of myocarditis by detecting Cox B virus IgM antibodies.
Objective:
To detect Coxsackie B virus type 1-6 IgM antibodies from 193 clinically diagnosed myocarditis cases.
Methods:
The capture IgM ELISA (Mac ELISA) test was used.
Results:
The total positive rate of Coxsackie virus B 1-6 IgM antibody in myocarditis patients was 69.6% (135 cases), among which Cox B3 type was as high as 40% (54 cases) of the total positive patients. The others were 29.6% (40 cases), 18.5% (25 cases), 7.4%(10 cases), 3.0% (4 cases), 1.5% (2 cases) of Cox B2, Cox B4, Cox B1, Cox B5 and Cox B6 respectively. Meanwhile only 20.4% (37 cases) Cox B IgM positive cases could be detected in 181 non-myocarditis patients of same ages. The results showed that the positive rate of Cox B virus IgM antibody in clinically diagnosed myocarditis was significantly higher than that in control group (P <0.01).
Conclusions:
The Cox B viruses may be the main agents causing viral myocarditis in Beijing cases. Mac ELISA is an easy, rapid, specific and sensitive method to detect Cox B virus IgM antibody for early and rapid diagnosis of myocarditis.
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