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[Cloning and expression of TTV gene fragment in E. coil and identification of the expressed protein]
Objective:
To construct a recombinant expression vector containing TTV gene fragment and identify the expressed protein.
Methods:
Based on computer-aided studies, TTV ORF12 gene fragment coding protein of antigenicity was amplified by PCR from the patient serum with non-A-G hepatitis. Then the gene fragment was integrated into the multiple cloning site of pQE-30 and expressed in E coil. The expressed protein was identified by ELISA.
Results:
The relative molecular weight of the expressed protein matches the predication and its yield was up to 22.33% of total bacterial proteins of E. coli The expressed protein was detected by ELISA.
Conclusions:
The recombinant vector has been constructed successfully and the expressed protein possesses antigenicity.
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