Related Experiment Video
Updated: Oct 5, 2026
Detection of Rabies IgG and IgM Antibodies Using the Rabies Indirect Fluorescent Antibody Test
Published on: January 19, 2024
[Detection for anti-Hantavirus IgM in patient serum with silver enhanced dot immunogold filtration assay]
1Third Affiliated Hospital of Xinxiang Medical College, Xinaiang 453003, China.
Objective:
To establish a more rapid and simple method for detecting anti-Hantavirus IgM in patient' s serum.
Methods:
A silver enhanced dot immunogold filtration assay (SEDIGFA) was developed by coating the nucleocapsid protein of Hantavirus on nitrocellulose (NC) membrane, adding colloidal gold labeled sheep anti-human IgM antibody and the silver enhancer. The whole procedure can be finished within 10 minutes.
Results:
The coincidence rate of the results of serum samples simultaneously operated with ELISA and SEDEIGFA was 98% for anti-Hantavirus IgM.
Conclusions:
SEDIGFA is more simple and rapid for application to small medical laboratories. It can be applied for early diagnosis of hemorrhagic fever with renal syndrome (HFRS).
