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[Culture of chondrocytes using allogenous acellular cartilaginous matrix]
1Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100041.
Objective:
To observe the effect of chondrocyte culture with natural ECM made from allogenous acellular cartilage.
Methods:
The allogenous acellular cartilaginous matrix was used as a scaffold in chondrocyte culture in vitro, comparing with the acellular perichondrium. The growth and regeneration of the chondrocytes in vitro were observed.
Results:
The chondrocytes seeded on the acellular cartilaginous matrix grew well while the chondrocytes on the acellular perichondrium matrix grew poorly.
Conclusion:
The surface condition of the scaffold has a notable influence on the growth of the cells. The allogenous acellular cartilaginous matrix may provide a suitable condition for chondrocytes, and it has the possibility to be developed as a natural scaffold for tissue engineering.