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Updated: Oct 5, 2026
A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk
Published on: November 7, 2019
[Human preadipocyte culture and the establishment of hyperplasia and hypertrophy model]
1Department of Plastic and Reconstructive Surgery, Second Military Medical University, Shanghai 200003.
Objective:
To better understand hyperplasia and hypertrophy properties of human adipose tissue.
Methods:
Using an "adhesion-ceiling" culture strategy, fibroblast-like cells from adult pure adipose granules were successfully cultured.
Results:
The cells were highly homogeneous, highly proliferative and with a high differentiation rate. Their dynamic morphological changes, growth curve, glycerophosphate dehydrogenase pattern, oil red O staining, and reaction to insulin and dexamethasone all verified their preadipocyte identity. Under controlled conditions, the preadipocytes replayed their in vivo hyperplasia and hypertrophy process.
Conclusion:
In mature human adipose tissue there exist functionally active fractions that can be modified. This study laid the basis for further probing into obesity and fat autotransplantation.
