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[Sequence analysis of the 5' and 3' terminal regions of dengue type 2 virus 04 strain]
1Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100850, China.
Objective:
To analyze the sequences of 5' and 3' terminal region of dengue type 2 virus 04 strain.
Methods:
Total RNA was isolated from dengue type 2 virus 04 strain (D2-04) infected C6/36 cells. With this RNA as template, the cDNA of both 5' and 3' termini of D2-04 were amplified using RACE method respectively. The cDNAs were separately inserted into pGEM-T vector and the recombinant plasmids containing the 5' terminus 535 bp and 3' terminus 503 bp were obtained. The nucleotide sequences of the inserted cDNA fragment were determined. The nucleotide sequences of the 5' and 3' noncoding regions of D2-04 were compared with other dengue type 2 viruses such as JAM, NGC, S1, 16681 and PD-53.
Results:
The results showed that they shared 98.96%, 98.96%, 93.75%, 98.95%, 97.92% and 97.72%, 97.80%, 90.65%, 94.26%, 94.22% homology with D2-04 strain respectively.
Conclusions:
Except SI, the homology between D2-04 and other type 2 viruses was higher than 94%.
