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[Mechanism of Se-Indocalamus tessdatus polycassine(S-ITPS) against human immunodeficiency virus type 1 (HIV-1)]
1The Center for AIDS Prevention and Control, Chinese Academy of Preventive Medicine, Beijing 100050, China.
Objective:
To study the mechanism of Se- Indochalamus tessdatus polycassine (S-ITPS) against HIV-1 and to provide a theoretical support for developing the anti-HIV drugs.
Methods:
S-ITPS was added to MT4 cells before viral inoculation, at the same time of viral inoculation, then the cells were cultured with or without S-ITPS for 1-4 weeks, finally, cytopathic effect (CPE), MTT staining method for viable cells(MTT assay),p24 antigen titer (ELISA), or infectivity (TCID50 assay) of the cultural supernatants were used as markers to monitor the virus growth.
Results:
S-ITPS can inhibit HIV-induced CPE (1C50 = l0microg/ml)and viral replication (1C50 = 156 microg/ml)in dose dependent manner. The virus infectivity in the presence of S-ITPS was greatly decreased than that of the control. The virus inhibition was enhanced under the presence of noncytotoxic drug concentration of <1250 microg/ml in cell culture, inhibition of viral replication by S-ITPS was 73.7%, 63.5%, 87.7% and 95.4% at week 1, week 2, week 3 and week 4 of cultured cells respectively. It can also inhibit absorption of HIV-1 to MT-4 cells, but no inhibition of HIV-1 was seen when MT-4 cells were pretreated with S-ITPS. Virus can be inactivated by the agent also.
Conclusions:
S-ITPS is a potent anti-HIV- 1 agent in MT-4 /HIV- 1 cultural system, at least two mechanisms were included inhibition both of HIV-1 absorption to MT-4 cells and viral replication in HIV-infected cells. S-ITPS inhibition of CPE is stronger than that of p24 antigen production.