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Updated: Aug 15, 2026
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
[Cloning and sequencing of complete TT virus genome from Beijing isolate of China]
Objective:
To obtain the complete TT virus genome from a Beijing isolate of China.
Methods:
PCR was performed for amplifying the TT virus genome from serum of a patient with non A-G hepatitis. Five overlapped fragments, that are 199 bp (1-199), 1 267 bp (90-1356), 878 bp (1354-2231),1129 bp(2178-3306),'434 bp(3306-3739), respectively, were obtained. These PCR products were sequenced by standard method.
Results:
The complete TT virus genome of Beijing isolate consists of 3 739 nucleotides involving two ORFs. The first ORF locates at 589-2902 nt, encoding 770 amino acids, the second ORF locates at 107-715 nt, encoding 202 amino acids. 5' and 3' terminus of the genome are non-coding regions including 106 bp and 837 bp respectively. In this genome, A is 31.2%, C 25.4%, G 22.5% and T 20.9%. The homology of Beijing isolate and Japanese TT virus was more than 95% at both nucleotide and amino acid levels.
Conclusions:
We successfully cloned and sequenced the complete TT virus genome from a Chinese patient. This Beijing TT virus isolate and that reported by Japan all belong to a same genotype.
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