Related Experiment Video
Updated: Aug 17, 2026
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
[Generation of monoclonal antibody Fab fragments to parathyroid hormone related protein by phage display technology]
1Institute of Virology, Chinese Academy of Preventive Medicine, Beining 100052, China.
Objective:
To develop monoclonal antibody Fab fragments using display technique.
Methods:
The mouse IgG Fab genes of heavy and light chains were amplified from spleen cells of a parathyroid hormone-related protein (PTHrP) immunized mouse. The combinatorial phage antibody library was prepared by inserting both heavy and light chain Fab genes into phagemid vector pComb3 and followed by infection of helper phage. The library was selected by purified recombinant PTHrP.
Results:
The combinatorial phage antibody library was constructed successfully and the specific mouse Fabs to PThrP were selected and expressed in E. coli.
Conclusions:
The selected specific mouse Fabs can recognize PTHrP with high specificity.
