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[Preparation of polyclonal antibody to human prion protein using the expressed GST-PrP fusion protein as antigen]
1Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Objective:
Preparing specific antibody to prion protein.
Methods:
Using prokaryotic expressed GST-PrP fusion protein as antigen, rabbits were immunized subcutaneously.
Results:
ELISA assay revealed that the titer of the prepared antiserum against expressed PrP protein was as high as 1:128 000. Western blot test showed that the antiserum was able to react with the in vitro expressed intact and different lengths of C-terminus truncated PrP proteins, as well as the native PrP proteins from brain homogenization of human and mouse.
Conclusions:
The prokaryotic expressed GST-PrP fusion protein can efficiently elicit in immunized animals the PrP-specific antibody.
