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Polymeric lamellar substrate particles for intranasal vaccination
I Jabbal-Gill1, W Lin, O Kistner
1West Pharmaceutical Services Drug Delivery and Clinical Research Centre Ltd., Albert Einstein Centre, Nottingham Science & Technology Park, University Boulevard, NG7 2TN, Nottingham, UK.
Advanced Drug Delivery Reviews
|August 23, 2001
Summary
Novel polymeric lamellar substrate particles (PLSP) offer a new method for vaccine antigen adsorption, preserving antigen integrity. This approach avoids harsh conditions, enhancing potential for effective nasal vaccines.
Area of Science:
- Vaccinology
- Biomaterials Science
- Nanotechnology
Background:
- Current vaccine development explores new antigens, adjuvants, and delivery routes, including mucosal vaccination.
- Biodegradable microparticles like poly(D,L-lactide) (PLA) and poly(D,L-lactide-co-glycolide) (PLGA) are studied for controlled vaccine release.
- Concerns exist regarding antigen integrity and immunogenicity during microparticle encapsulation due to solvents, shear stress, and low pH.
Purpose of the Study:
- To introduce polymeric lamellar substrate particles (PLSP) as a novel system for antigen adsorption.
- To discuss factors influencing PLSP characteristics and antigen adsorption onto PLSP.
- To evaluate the potential of PLSP as adjuvants for nasal delivery of protein, peptide, or viral vaccines.
Main Methods:
- PLSP are produced via simple precipitation of poly(D,L-lactide) (PLA).
- Antigens are adsorbed onto PLSP, avoiding organic solvents and extreme pH conditions.
- Factors affecting PLSP characteristics and antigen adsorption are discussed.
Main Results:
- PLSP formation by precipitation preserves antigen integrity by avoiding harsh encapsulation conditions.
- This method circumvents antigen exposure to organic solvents, high shear stress, and low pH.
- PLSP demonstrate potential for effective antigen adsorption and preservation.
Conclusions:
- PLSP represent a promising alternative to traditional microparticle systems for vaccine delivery.
- The adsorption method maintains antigen integrity, crucial for immunogenicity.
- PLSP hold potential as adjuvants for nasal vaccine formulations, particularly for protein, peptide, and viral antigens.