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Related Experiment Videos

Single-cell internalization during zebrafish gastrulation.

A Carmany-Rampey1, A F Schier

  • 1Developmental Genetics Program, Skirball Institute of Biomolecular Medicine and Department of Cell Biology, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA.

Current Biology : CB
|August 30, 2001
PubMed
Summary
This summary is machine-generated.

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In zebrafish gastrulation, the protein One-eyed pinhead (Oep) is crucial for cell internalization. Single cells lacking Oep can still internalize and form mesendoderm when in a wild-type environment, suggesting autonomous cell behavior.

Area of Science:

  • Developmental Biology
  • Cellular Biology
  • Genetics

Background:

  • Gastrulation establishes germ layers, but cellular interactions are poorly understood.
  • Nodal signaling, involving Cyclops, Squint, and One-eyed pinhead (Oep), is vital for zebrafish mesendoderm formation.
  • Maternal-zygotic oep (MZoep) mutants exhibit failed cell internalization during gastrulation.

Purpose of the Study:

  • To investigate whether cell internalization during zebrafish gastrulation is a cell-autonomous process or requires group interactions.
  • To determine the role of One-eyed pinhead (Oep) in cell-autonomous mesendoderm formation.

Main Methods:

  • Analysis of cell internalization in maternal-zygotic oep (MZoep) mutant zebrafish embryos.
  • Cell transplantation experiments: MZoep mutant cells into wild-type embryos and wild-type cells into MZoep mutant embryos.

Related Experiment Videos

  • Assessment of mesendodermal marker gene expression (axial/foxA2, sox17) in transplanted cells.

    • Main Results:

    • MZoep mutant cells fail to internalize and adopt neural/tail fates when developing normally.
    • Individual MZoep mutant cells transplanted to wild-type margins internalize but do not contribute to mesendoderm.
    • Single wild-type cells transplanted to MZoep mutant margins autonomously internalize and express mesendodermal markers.

    Conclusions:

    • Cell internalization and mesendoderm specification during zebrafish gastrulation can occur autonomously in single cells.
    • The cofactor One-eyed pinhead (Oep) plays a cell-autonomous role in Nodal signaling required for mesendoderm formation.
    • These findings clarify the cellular mechanisms underlying germ layer formation in vertebrate development.