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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Related Experiment Video

Updated: May 27, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

MicroRNA function and mechanism: insights from zebra fish.

A F Schier1, A J Giraldez

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

Cold Spring Harbor Symposia on Quantitative Biology
|March 27, 2007
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) are key regulators of gene expression. In zebrafish, miR-430 is essential for development, promoting mRNA decay and facilitating developmental transitions by targeting hundreds of mRNAs.

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Microinjection of Zebrafish Embryos to Analyze Gene Function
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Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
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Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

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Related Experiment Videos

Last Updated: May 27, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

Microinjection of Zebrafish Embryos to Analyze Gene Function
07:18

Microinjection of Zebrafish Embryos to Analyze Gene Function

Published on: March 9, 2009

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
05:48

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Published on: May 23, 2013

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression post-transcriptionally.
  • miRNAs function by binding to the 3' untranslated region (UTR) of target messenger RNAs (mRNAs).
  • Zebrafish serve as a powerful model organism for studying developmental processes due to their rapid external development.

Purpose of the Study:

  • To investigate the developmental roles of miRNAs in zebrafish.
  • To elucidate the mechanisms by which miRNAs regulate target mRNAs during development.
  • To determine the necessity of miRNA processing for embryonic development.

Main Methods:

  • Generation of zebrafish embryos lacking the Dicer enzyme, a key component of miRNA processing.
  • Analysis of developmental phenotypes in Dicer-deficient zebrafish embryos.
  • Rescue experiments involving the injection of specific miRNAs (miR-430) into Dicer mutant embryos.
  • Assessment of maternal mRNA decay and deadenylation in response to miRNA activity.

Main Results:

  • Zebrafish embryos lacking Dicer are devoid of mature miRNAs and exhibit significant morphogenesis defects.
  • Despite the absence of mature miRNAs, Dicer mutants can still differentiate multiple cell types.
  • Injection of miR-430 miRNAs rescues the early morphogenesis defects observed in Dicer mutants.
  • miR-430 was shown to accelerate the decay of hundreds of maternal mRNAs and induce deadenylation of target mRNAs.

Conclusions:

  • miRNAs, specifically miR-430, play a crucial role in facilitating developmental transitions in zebrafish.
  • miRNAs are not essential for all cell fate specification or signaling pathways but are important for regulating mRNA stability.
  • The study demonstrates that miRNAs induce deadenylation and decay of a large number of target mRNAs during early development.