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Updated: Aug 8, 2026
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Measuring Local Anaphylaxis in Mice
Published on: October 14, 2014
Contact allergenic potency: correlation of human and local lymph node assay data
G F Gerberick1, M K Robinson, C A Ryan
1Human and Environmental Safety Division, Procter and Gamble, Cincinnati, OH 45252, USA. gerberick.gf@pg.com
Summary
The murine local lymph node assay (LLNA) accurately predicts chemical skin sensitization potency. LLNA EC(3) values closely correlate with human no-effect levels, aiding in allergic contact dermatitis risk assessment.
Area of Science:
- Toxicology
- Dermatology
- Risk Assessment
Background:
- Assessing skin sensitization potential is crucial for identifying contact allergens and evaluating risks.
- Allergic contact dermatitis (ACD) is caused by sensitizing chemicals, with dose-response relationships and thresholds influencing hazard.
- The murine local lymph node assay (LLNA) is a key preclinical method for sensitization hazard assessment.
Purpose of the Study:
- To compare potency estimations from LLNA data with clinical human data.
- To validate the LLNA's utility in predicting relative skin sensitization potency.
Main Methods:
- Determined no-effect levels (NOELs) for 21 chemicals from human repeat patch test studies.
- Compared human NOELs with LLNA EC(3) values, representing the concentration for a 3-fold increase in lymph node cell activity.
Main Results:
- Chemicals were classified by potency (strong to nonsensitizing) using human data and LLNA EC(3) values.
- LLNA EC(3) values showed strong comparability with human NOELs.
- Potency rankings derived from LLNA data supported human classifications.
Conclusions:
- The LLNA provides quantitative estimates of relative skin sensitization potency.
- LLNA EC(3) values closely correlate with NOELs from human repeat patch testing.
- The LLNA is a reliable tool for assessing skin sensitization potency in risk assessments.
