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Derivatization and template-guided ligation of oligodeoxyribonucleotides using cyanogen bromide and N-substituted
Z A Shabarova1, O A Fedorova, N G Dolinnaya
1Chemistry Department, Lomonosov Moscow State University, Russia.
Abstract:
Cyanogen bromide has been found to induce the template-guided condensation of oligonucleotides only in the presence of N-substituted morpholines. Based on 31P, 1H and 13C NMR spectroscopy data, the mechanism of the phosphomonoester group activation by cyanogen bromide in N-substituted morpholine buffers is suggested. It has also been shown that BrCN can be used for the synthesis of oligonucleotide derivatives in aqueous solution.
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