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Influence of buoyancy-driven convection on protein separation by free-flow electrophoresis
M J Clifton1, N Jouve, V Sanchez
1Laboratoire de Génie Chimique et Electrochimie, Université Paul Sabatier, Toulouse, France.
Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)
|January 1, 1992
Abstract:
In free-flow electrophoresis, the stability and reproducibility of the flow field are prerequisites for a satisfactory separation. Due to the presence of the species to be separated and due to mass- and heat-transfer phenomena, non-uniformities in density inevitably appear, both within the carrier buffer and between the carrier buffer and the sample stream. On earth they give rise to buoyancy-driven convection which interferes with the separation. These effects have been quantified by numerical modelling. Agreement has been found between the numerical results and experimental observations.