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[Expression of hepatitis A virus recombinant proteins in Escherichia coli]
V A Ternovoĭ1, T Iu Bondarenko, V B Bondarchuk
1Institute of Molecular Biology-State Research Center Vector, Koltsovo, Novosibirsk Region, Russia.
Abstract:
On the basis of coding regions on the fragments of genes P1 and P2 of hepatitis A virus (HAV) recombinant proteins of this virus have been synthesized in the prokaryotic expressing system of E. coli, isolated and studied with the use of sera obtained from hepatitis A patients. The capacity of HAV recombinant proteins for binding with the sera of patients with hepatitis A in the acute stage has been shown with the use of immunoblotting and the indirect solid-phase enzyme immunoassay. The results obtained in this investigation are discussed in the light of the possible use of recombinant proteins for the detection of HAV markers.