Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation and structural characterization of colistin components.

J A Orwa1, C Govaerts, R Busson

  • 1Katholieke Universiteit Leuven, Faculteit Farmaceutische Wetenschappen, Belgium.

The Journal of Antibiotics
|September 19, 2001
PubMed
Summary

Researchers isolated and identified six colistin components using preparative chromatography and NMR. Two novel polymyxin structures, Polymyxin E7 and Isoleucine Polymyxin E8, were characterized for the first time, expanding knowledge of these important antibiotics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Double burden: financial toxicity in patients with advanced soft tissue sarcoma at the start of first-line palliative chemotherapy: baseline data from the HOLISTIC study.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2025
Same author

Setting the international research agenda for sarcomas with patients and carers: results of phase II of the Sarcoma Patient Advocacy Global Network (SPAGN) priority setting partnership.

BMC cancer·2024
Same author

Sunitinib for the treatment of metastatic gastrointestinal stromal tumors: the effect of TDM-guided dose optimization on clinical outcomes.

ESMO open·2024
Same author

Study of tocopherol content and its potential antioxidant activity in commercial lipid emulsions for parenteral nutrition.

Die Pharmazie·2020
Same author

Influence of the solvent system on the stability of doxycycline solutions.

Journal of pharmaceutical and biomedical analysis·2018
Same author

Chiral separations by non-aqueous capillary electrophoresis in DMSO-based background electrolytes.

Talanta·2013

Area of Science:

  • Analytical Chemistry
  • Organic Chemistry
  • Pharmacology

Background:

  • Colistin is a critical antibiotic for treating multidrug-resistant Gram-negative bacterial infections.
  • Characterization of colistin components is essential for understanding its activity and developing new analogs.
  • Previous studies have identified several colistin congeners, but novel structures continue to emerge.

Purpose of the Study:

  • To isolate and characterize individual components from a bulk colistin sulphate sample.
  • To determine the structures of previously unidentified colistin congeners.
  • To contribute to the understanding of colistin's chemical diversity and structure-activity relationships.

Main Methods:

  • Preparative-scale high-performance liquid chromatography (HPLC) using a poly(styrene-divinylbenzene) stationary phase.

Related Experiment Videos

  • Isocratic elution with a specific acetonitrile-sodium sulphate-water mobile phase.
  • Structural elucidation using Nuclear Magnetic Resonance (NMR) spectroscopy (1H and 13C) and Mass Spectrometry (MS).

    • Main Results:

    • Six distinct colistin components were successfully isolated from the bulk sample.
    • The molecular weights of all isolated components were confirmed by mass spectrometry.
    • The structures of two novel colistin components, Polymyxin E7 and Isoleucine Polymyxin E8, were determined for the first time.
    • Polymyxin E7 differs from Polymyxin E1 by possessing a 7-methyloctanoic acid fatty acid moiety.
    • Isoleucine Polymyxin E8 is characterized by a 7-methylnonanoic acid fatty acid moiety, distinguishing it from isoleucine Polymyxin E1.

    Conclusions:

    • Preparative chromatography is an effective method for separating complex mixtures of colistin congeners.
    • The identification of Polymyxin E7 and Isoleucine Polymyxin E8 highlights the structural diversity within the colistin family.
    • These findings provide valuable insights into the chemical structures of colistin components, aiding in the development of new antimicrobial agents.