Multiple oligonucleotide synthesis in tandem on solid-phase supports for small and large scale synthesis
1Dept. Biochem. & Molec. Biol., University of Calgary, Calgary, AB, Canada T2N 4N1.
Nucleosides, Nucleotides & Nucleic Acids
|September 21, 2001
Abstract:
Multiple oligonucleotides linked end-to-end in tandem can be synthesized by adding a nucleoside to the 5'-OH end of a prior sequence. Nucleosides with 3'-succinyl or Q-Linker arms are coupled with HBTU/DMAP. Alternatively, new phosphoramidite reagents with 3'-ester linkages can be used. Hydroxyl or amino supports can also be used as universal starting materials. Treatment with NH4OH cleaves the 3'-ester to yield only 3'-OH groups and no unwanted 3'-phosphorylated products occur.
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