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Updated: Aug 2, 2026

Enzymatic Cascade Reactions for the Synthesis of Chiral Amino Alcohols from L-lysine
Published on: February 16, 2018
Effect of mobile phase acidic additives on enantioselectivity for phenylalanine analogs
1DuPont Pharmaceutical Company, Chemical Process Research and Development, Deepwater, NJ 08023, USA. yun.k.ye@dupontpharma.com
Abstract:
The use of acidic mobile phase additives allows the chiral separation of underivatized phenylalanine analogs on a common amylosic column. In addition to decreasing retention and band-broadening arising from non-ideal interactions, acidic additives may also increase selectivity. This appears to be due to the minimization of non-selective binding in the recognition site. Effects of the additives are related to additive pKa and size. Ethanesulfonic acid was typically the most effective additive with trifluoroacetic acid being one of the least effective.
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