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Updated: Jul 17, 2026

Genetic Modification and Recombination of Salivary Gland Organ Cultures
Published on: January 28, 2013
Strand-specific postreplicative processing of mammalian telomeres
S M Bailey1, M N Cornforth, A Kurimasa
1Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM 87544, USA.
Abstract:
Telomeres are specialized nucleoprotein structures that stabilize the ends of linear eukaryotic chromosomes. In mammalian cells, abrogation of telomeric repeat binding factor TRF2 or DNA-dependent protein kinase (DNA-PK) activity causes end-to-end chromosomal fusion, thus establishing an essential role for these proteins in telomere function. Here we show that TRF2-mediated end-capping occurs after telomere replication. The postreplicative requirement for TRF2 and DNA-PKcs, the catalytic subunit of DNA-PK, is confined to only half of the telomeres, namely, those that were produced by leading-strand DNA synthesis. These results demonstrate a crucial difference in postreplicative processing of telomeres that is linked to their mode of replication.
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