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[Endogenous substance P in corneal epithelial cells and keratocytes]
M Watanabe1, K Nakayasu, M Iwatsu
1Eye Department, Asama Municipal Hospital.
Nippon Ganka Gakkai Zasshi
|October 3, 2001
Abstract
Purpose:
To detect endogenous substance P(SP) and neurokinin receptor 1(NK1R) in cultured human epithelial cells of the cornea(HE) and human keratocytes(HK).
Method:
Messenger RNA(mRNA) expression of SP and endogenous SP in HE and HK were detected by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). mRNA expression of NK1R in HE and HK was detected by RT-PCR.
Results:
The mRNA expression of SP and endogenous SP were recognized in HE and HK. The mRNA of NK1R was also expressed in HE and HK.
Conclusion:
It appears that endogenous SP regulates the biological functions of HE and HK in an autocrine or paracrine fashion.