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Quantifying spatial localization of optical mapping using Monte Carlo simulations.

L Ding1, R Splinter, S B Knisley

  • 1Department of Electrical Engineering, Georgia Institute of Technology, Atlanta 30332, USA.

IEEE Transactions on Bio-Medical Engineering
|October 5, 2001
PubMed
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Optical mapping techniques for cardiac activity reveal that signal localization depends on tissue optics and imaging method. Both broad-field and laser scanning methods collect signals from larger tissue areas than expected.

Area of Science:

  • Biomedical Optics
  • Cardiovascular Physiology
  • Biophysics

Background:

  • Optical mapping techniques visualize cardiac electrical and calcium activity using fluorescent dyes.
  • Two main methods exist: broad-field excitation and laser scanning.
  • The spatial origin of the fluorescence signal in these methods is not fully understood.

Purpose of the Study:

  • To investigate how light absorption and scattering in cardiac tissue affect spatial localization in optical mapping.
  • To compare signal origins for broad-field versus laser scanning optical mapping methods.

Main Methods:

  • Measured optical properties (absorption, scattering, anisotropy) of rabbit heart tissue with voltage/calcium dyes.
  • Employed Monte Carlo simulations to model light propagation and fluorescence emission.

Related Experiment Videos

  • Quantified contributions of local tissue emissions to surface-collected fluorescence for both mapping methods.

    • Main Results:

    • Spatial localization of fluorescence signals is significantly influenced by tissue optical properties.
    • Both broad-field and laser scanning methods collect signals from tissue regions larger than the excitation source or detector area.
    • The specific optical mapping technique impacts the spatial fidelity of the recorded signals.

    Conclusions:

    • Tissue absorption and scattering critically determine spatial accuracy in optical mapping.
    • Understanding these optical effects is essential for interpreting cardiac activity maps.
    • The choice of optical mapping method influences the effective region contributing to the recorded signal.