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Downstream processing of enzymatically produced geranyl glucoside.

B M de Roode1, L Oliehoek, A van der Padt

  • 1Department of Agrotechnology and Food Sciences, Food and Bioprocess Engineering Group, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.

Biotechnology Progress
|October 6, 2001
PubMed
Summary
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Enzymatic production of geranyl glucoside was optimized using a spray column reactor. Adsorption onto alumina proved successful for downstream processing, yielding a pure product efficiently.

Area of Science:

  • Biotechnology and biochemical engineering
  • Enzyme technology and biocatalysis
  • Separation science and downstream processing

Background:

  • Geranyl glucoside, a derivative of geraniol, is valuable in the fragrance and flavor industry.
  • Its "slow release" aroma properties necessitate efficient production and purification methods.
  • Investigating enzymatic synthesis and downstream processing is crucial for its industrial application.

Purpose of the Study:

  • To investigate the enzymatic production of geranyl glucoside.
  • To evaluate and optimize downstream processing methods for geranyl glucoside recovery.
  • To assess the feasibility of large-scale production using the developed system.

Main Methods:

  • Enzymatic production of geranyl glucoside in a spray column reactor.

Related Experiment Videos

  • Utilized a pretreated hydrophobic microfiltration membrane to separate phases.
  • Evaluated four downstream processing techniques: water extraction, foaming, alumina adsorption, and reduced pressure distillation.

    • Main Results:

    • Achieved an initial production rate of 0.58 mg x U(-1) x h(-1) for geranyl glucoside.
    • Alumina adsorption successfully recovered geranyl glucoside with a maximum adsorption capacity of 7.86 mg x g(-1).
    • Distillation yielded an impure product due to co-present glucose; water extraction and foaming were unsuitable.

    Conclusions:

    • Enzymatic production in a spray column reactor coupled with alumina adsorption is an effective method for geranyl glucoside purification.
    • The developed system demonstrates potential for large-scale production, with 1 kg achievable in 2 days.
    • Optimized downstream processing is key to realizing the industrial potential of geranyl glucoside.