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Endoluminal smooth muscle cell seeding limits intimal hyperplasia.
D Gomes1, L Louedec, D Plissonnier
1U460 Inserm, CHU Xavier Bichat, Paris, France.
Journal of Vascular Surgery
|October 23, 2001
Summary
Seeding vascular smooth muscle cells (SMCs) into injured rat aortas significantly reduced intimal hyperplasia. This cell-seeding strategy limits vascular wall thickening in both mechanical injury and allograft rejection models.
Area of Science:
- Vascular Biology
- Regenerative Medicine
- Transplantation Immunology
Background:
- Intimal hyperplasia is a primary vascular wall response to injury.
- It is a major cause of graft failure and vascular disease.
Purpose of the Study:
- To test if endoluminal seeding of host syngeneic vascular cells can limit intimal hyperplasia.
- To evaluate this in models of mechanical deendothelialization and chronic allograft rejection in rat aorta.
Main Methods:
- In situ seeding of syngeneic endothelial cells, smooth muscle cells (SMCs), and fibroblasts (FIBs) in mechanically deendothelialized and allografted rat aortas.
- Evaluation of cell adherence and intimal hyperplasia using histologic and morphometric methods at 2 months.
Main Results:
- SMCs and FIBs adhered to the deendothelialized aortic wall; endothelial cells did not.
- SMC seeding significantly reduced intimal thickness and nuclear content in both mechanical injury and allograft models compared to controls or FIB seeding.
- SMC-seeded intimal hyperplasia was richer in elastin, while FIB-seeded hyperplasia was richer in collagen.
Conclusions:
- Endoluminal seeding of syngeneic SMCs effectively reduces intimal hyperplasia in rat aorta models.
- Cellular seeding influences extracellular matrix accumulation in the neointima.