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An enhancer sequence directs LacZ expression to developing pharyngeal endoderm in transgenic mice
Genesis (New York, N.Y. : 2000)
|October 23, 2001
Summary
Researchers identified a novel enhancer in the Hoxc-6 gene
Area of Science:
- Developmental biology
- Genetics
- Molecular biology
Background:
- The murine Hoxc-6 homeobox gene has two promoters, PRI and PRII.
- The PRII promoter region was sequenced, revealing an Antananapedia-like consensus binding sequence.
Discussion:
- A LacZ reporter gene construct with three copies of this sequence showed specific expression in pharyngeal endoderm cells of transgenic mice.
- Expression was detected early in development (E7.5) and became prominent by E9.5 in the pharyngeal endoderm.
Key Insights:
- The identified enhancer directs specific expression in the developing pharyngeal endoderm.
- Colocalization studies confirmed expression with key endodermal genes like HNF3beta, Pax-9, Shh, and Nkx2.5.
Outlook:
- This novel enhancer serves as a valuable tool for tracking the morphogenetic movements of foregut precursor cells.
- Further research can utilize this enhancer to study cell fate and patterning in the developing foregut.