Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A basic peroxidase from wheat kernel with antifungal activity.

C Caruso1, G Chilosi, L Leonardi

  • 1Dipartimento di Agrobiologia e Agrochimica Università della Tuscia, Via S. Camillo De Lellis, I-01100, Viterbo, Italy. caruso@unitus.it

Phytochemistry
|October 24, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical evaluation of corneal elasticity and true IOP using a dual-applanation tonometer.

Frontiers in ophthalmology·2026
Same author

Living with chronic rhinosinusitis with nasal polyposis (CRSwNP): an experience to amplify Italian patients' voices.

Journal of patient-reported outcomes·2026
Same author

Eosinophilic ascites and eosinophilic gastrointestinal diseases.

European annals of allergy and clinical immunology·2025
Same author

Inflammatory signatures and immunomodulation in neonates: a pilot study.

La Clinica terapeutica·2025
Same author

Reply to Correspondence: The Bronchodilator and Anti-Inflammatory Effect of Long-Acting Muscarinic Antagonists in Asthma: An EAACI Position Paper.

Allergy·2025
Same author

The Bronchodilator and Anti-Inflammatory Effect of Long-Acting Muscarinic Antagonists in Asthma: An EAACI Position Paper.

Allergy·2024
Same journal

Five undescribed compounds isolated from Gerbera delavayi with their anti-inflammatory activity.

Phytochemistry·2026
Same journal

Ingenane diterpenoids with anti-inflammatory activity from the whole plants of Euphorbia peplus.

Phytochemistry·2026
Same journal

Discovery of cytotoxic 1,4-benzodioxane oxyneolignan analogues from Glechoma longituba.

Phytochemistry·2026
Same journal

Cinnamolides A-G, seven previously undescribed phytoconstituents from the peels of Cinnamomum chago and their anti-inflammatory activity.

Phytochemistry·2026
Same journal

Antiviral amide derivatives from Uvaria siamensis.

Phytochemistry·2026
Same journal

COX-2 inhibitors from Laportea bulbifera: Structure-activity relationship, kinetic investigation, and molecular docking.

Phytochemistry·2026
See all related articles

A novel wheat heme-peroxidase (WP1) exhibits antifungal activity against plant pathogens. This discovery suggests peroxidases play a crucial role in plant defense mechanisms, offering potential for agricultural applications.

Area of Science:

  • Biochemistry
  • Plant Pathology
  • Allergen Research

Background:

  • Heme-peroxidases are enzymes involved in various biological processes.
  • Wheat flour contains allergens implicated in baker's asthma.
  • The specific functions of many plant peroxidases remain underexplored.

Purpose of the Study:

  • To purify and characterize a basic heme-peroxidase (WP1) from wheat.
  • To investigate the enzymatic and antifungal activities of WP1.
  • To explore the potential role of peroxidases in plant defense.

Main Methods:

  • Purification of WP1 from wheat kernels to homogeneity.
  • Biochemical characterization including molecular mass and isoelectric point determination.
  • Enzymatic assays for indole-3-acetic acid oxidase activity.

Related Experiment Videos

  • In vitro antifungal assays against phytopathogenic fungi.

    • Main Results:

    • WP1 was purified as a non-glycosylated 36 kDa protein with a pI of 8.0.
    • WP1 demonstrated indole-3-acetic acid oxidase activity.
    • WP1 significantly inhibited germ tube elongation in phytopathogenic fungi.

    Conclusions:

    • Wheat heme-peroxidase WP1 possesses significant antifungal properties.
    • This study provides evidence for the defensive role of peroxidases in plants.
    • WP1 may represent a novel target for managing plant fungal diseases.