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Microarray sampling-platform fabrication using bubble-jet technology for a biochip system
L R Allain1, M Askari, D L Stokes
1Life Sciences Division, Oak Ridge National Laboratory, TN 37831-6101, USA.
Fresenius' Journal of Analytical Chemistry
|October 27, 2001
Summary
This study presents a cost-effective method for creating DNA microarrays using inkjet printers. This biochip technology enables the detection of tumor suppressor genes like FHIT in mouse models.
Area of Science:
- Biotechnology
- Genomics
- Molecular Biology
Background:
- Microarray fabrication traditionally involves expensive spotting techniques.
- Inkjet printing offers a cheaper, mass-producible alternative for biological sample arraying.
Purpose of the Study:
- To describe a cost-effective method for fabricating DNA microarrays using a modified inkjet printer.
- To demonstrate the utility of this biochip platform for detecting specific genes, such as the tumor suppressor FHIT.
Main Methods:
- Genomic DNA from mouse tumors was amplified using PCR.
- Arrays were printed onto Zetaprobe membranes using a modified thermal inkjet printer.
- Hybridization assays with fluorescent probes were used for detection.
Main Results:
- Successful fabrication of microarrays containing PCR-amplified mouse tumor DNA.
- Demonstrated detection of the human fragile histidine triad (FHIT) gene using the developed biochip platform.
- The inkjet printing method proved to be a viable and economical alternative to conventional microarray printing.
Conclusions:
- Modified inkjet printing provides a simple, cost-effective approach for biochip microarray fabrication.
- This method is effective for detecting specific genes, including tumor suppressors, in biological samples.
- The developed biochip microarray platform shows promise for cancer research and diagnostics.