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Updated: Aug 2, 2026
17:14
In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
Biological basket weaving: formation and function of clathrin-coated vesicles
F M Brodsky1, C Y Chen, C Knuehl
1Department of Biopharmaceutical Sciences, University of California, San Francisco, California, USA. fmarbro@itsa.ucsf.edu
Annual Review of Cell and Developmental Biology
|November 1, 2001
Summary
Recent advances reveal how clathrin-coated vesicle (CCV) formation is regulated by novel proteins and molecular pathways. This complex regulation varies by cellular location and is crucial for tissue function and preventing disease.
Area of Science:
- Cell biology
- Molecular and structural biology
Background:
- Clathrin-coated vesicles (CCVs) are essential for intracellular transport.
- Understanding CCV formation and function is critical for cell biology.
Purpose of the Study:
- To summarize recent progress in understanding CCV formation and regulation.
- To highlight the role of novel regulatory proteins and molecular pathways.
Main Methods:
- Determination of CCV coat component structures at molecular resolution.
- Identification of novel regulatory proteins involved in CCV formation.
- Analysis of regulatory pathways including phosphorylation, receptor signaling, and lipid modification.
Main Results:
- CCV formation is driven by clathrin coat assembly but modulated by specific regulatory proteins.
- Regulation differs based on CCV location (plasma membrane, trans-Golgi network) and cell type.
- Cytoskeletal interactions and CCV transport pathways are increasingly understood.
Conclusions:
- Clathrin coat assembly is a fundamental process modified by diverse regulatory mechanisms.
- Cellular location and tissue-specific requirements dictate specialized CCV regulation.
- Dysregulation of CCV pathways is linked to human diseases.
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