Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stem cells from the Mammalian blastocyst.

J Rossant1

  • 1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5. rossant@mshri.on.ca

Stem Cells (Dayton, Ohio)
|November 20, 2001
PubMed
Summary

Mammalian embryonic stem cells (ES cells) and trophoblast stem cells (TS cells) are distinct cell types. Their potential is influenced by intrinsic regulators like Oct4 and extrinsic environmental factors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome Wide Conditional Mouse Knockout Resources.

Drug discovery today. Disease models·2024
Same author

Developmental differences in the expression of FGF receptors between human and mouse embryos.

Placenta·2014
Same author

Immunoregulation of host-versus-graft responses in the uterus.

Immunology today·2014
Same author

The missense mutation W290R in Fgfr2 causes developmental defects from aberrant IIIb and IIIc signaling.

Developmental dynamics : an official publication of the American Association of Anatomists·2010
Same author

Forward genetic screen of mouse reveals dominant missense mutation in the P/Q-type voltage-dependent calcium channel, CACNA1A.

Genes, brain, and behavior·2007
Same author

Genetic regulation of stem cell origins in the mouse embryo.

Clinical genetics·2005

Area of Science:

  • Developmental biology
  • Stem cell biology
  • Genetics

Background:

  • Mammalian embryonic development establishes two primary lineages: the inner cell mass (ICM) and trophectoderm.
  • ICM cells are pluripotent, forming fetal tissues, while trophectoderm cells form placental tissues.
  • Distinct stem cell lines, embryonic stem (ES) cells and trophoblast stem (TS) cells, can be derived from these lineages in mice.

Purpose of the Study:

  • To investigate the factors governing stem cell potential and lineage commitment in early mammalian embryos.
  • To understand the interplay between intrinsic regulators and extrinsic environmental cues in maintaining stem cell identity.

Main Methods:

  • Derivation and characterization of mouse ES and TS cell lines.
  • Analysis of gene expression, particularly the pluripotency regulator Oct4.
  • Chimeric analysis to assess cell fate in vivo.

Main Results:

  • ES and TS cells are morphologically and molecularly distinct, requiring different signaling pathways for maintenance.
  • Oct4 expression levels can influence ES cell fate towards a TS phenotype under specific conditions.
  • Stem cell potential is regulated by a combination of intrinsic factors and environmental signals.

Conclusions:

  • Stem cell identity and potential in early embryos are determined by both internal genetic factors and external environmental cues.
  • Manipulating these intrinsic and extrinsic regulators may unlock the potential of stem cells from various developmental stages and adult tissues.

Related Experiment Videos