Factors controlling embryo viability

Many diverse factors affect embryo viability. The morphological measures routinely used to grade human embryos are of limited use as many of the factors influencing the longterm viability of embryos are genetic or molecular and are undetectable or inconclusive from visualization of living embryos by microscopy. This article presents examples of factors that are known to affect embryo viability, including gamete formation, embryonic genome activation, and im-printing. Aspects of both gamete and embryo development are addressed, and the possibility that various anomalies remain hidden for extended periods before impacting upon a later aspect of development is hypothesized. In future, more detailed and informative assessments of embryo viability before embryo transfer may require invasive approaches to study the composition of embryos at various stages of preimplantation development; however, indirect, non-invasive measures would be preferable.