Evaluation of thin-layer methods in urine cytology

Conventional cytospin smears prepared from urinary tract specimens were compared with two new thin layer techniques, i.e. ThinPrep and AutoCyte PREP. Cellularity, cell preservation, background features, detection rate, screening time and ease of preparation were evaluated. Thin-layer techniques when applied to urine cytology were found to improve cell yield and cell preservation, and reduce background artefact. The reporting rate for abnormal urothelial cells was comparable to conventional cytospin smears, as was screening time. Laboratory staff found the methodologies to be practicable and easily incorporated into a large routine diagnostic service. We conclude that a one-slide thin-layer urine preparation is comparable to four cytospin slides in the detection of urothelial abnormalities, and that both ThinPrep and AutoCyte PREP have comparable features.