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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
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Published on: February 8, 2015

Cytoplasmic illuminations: in planta targeting of fluorescent proteins to cellular organelles

C Hawes1, C M Saint-Jore, F Brandizzi

  • 1Research School of Biological and Molecular Sciences, Oxford Brookes University, Oxford OX3 0BP, United Kingdom.

Protoplasma
|December 6, 2001
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...