Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA-PKcs is critical for telomere capping.

D Gilley1, H Tanaka, M P Hande

  • 1Department of Cellular and Molecular Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Proceedings of the National Academy of Sciences of the United States of America
|December 14, 2001
PubMed
Summary

DNA-dependent protein kinase catalytic subunit (DNA-PKcs) deficiency causes telomere fusions without affecting telomere length. This suggests DNA-PKcs plays a crucial role in mammalian telomere capping, distinct from telomere length maintenance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Analysis of posterior uterus approach combined with vascular occlusion in placenta percreta].

Zhonghua fu chan ke za zhi·2026
Same author

[Assessing the accuracy and comprehensiveness of large language models in responding to patient inquiries on placenta accreta spectrum].

Zhonghua yi xue za zhi·2025
Same author

[Analysis of clinical manifestations and genetic characteristics of a late-onset auditory neuropathy pedigree caused by a mitochondrial <i>MT-TS1</i> gene mutation m.7471dup].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2025
Same author

[Impact of the number of cesarean deliveries on adverse pregnancy outcomes of cesarean section in a single-center cohort study].

Zhonghua fu chan ke za zhi·2025
Same author

[Changes of hemodynamic parameters in normal pregnant women and their relationship with maternal basic characteristics].

Zhonghua fu chan ke za zhi·2024
Same author

[Clinical analysis of 86 cases of acute fatty liver of pregnancy].

Zhonghua fu chan ke za zhi·2023

Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is vital for DNA repair through nonhomologous end joining.
  • Severe combined immunodeficiency (SCID) mice exhibit telomere maintenance defects, linked to DNA-PKcs alterations.
  • The precise role of DNA-PKcs in telomere maintenance requires further investigation.

Purpose of the Study:

  • To investigate the specific role of DNA-PKcs in telomere maintenance using a knockout mouse model.
  • To differentiate the functions of DNA-PKcs in telomere capping versus telomere length maintenance.

Main Methods:

  • Generation and analysis of DNA-PKcs-deficient mice.
  • Culturing of primary mouse embryonic fibroblasts (MEFs) and kidney cells.
Keywords:
Non-programmatic

Related Experiment Videos

  • Assessment of telomere length, telomere fusions, chromosome fragments, and breaks.

    • Main Results:

    • DNA-PKcs-deficient cells show significant telomere fusions but maintain wild-type telomere length.
    • Elevated chromosome fragments and breaks were observed in DNA-PKcs-deficient cells.
    • The findings separate telomere capping and length maintenance phenotypes.

    Conclusions:

    • DNA-PKcs is essential for mammalian telomere capping.
    • The study elucidates a distinct role for DNA-PKcs in preventing telomere fusions.