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Axon voltage-clamp simulations. A multicellular preparation.

F Ramón, N Anderson, R W Joyner

    Biophysical Journal
    |January 1, 1975
    PubMed
    Summary
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    Simulating voltage clamp on nerve fiber bundles reveals that series resistance prevents accurate transmembrane potential control. Even with propagated action potentials, recorded currents lack characteristic notches, indicating limitations in multicellular preparations.

    Area of Science:

    • Neuroscience
    • Computational Biology
    • Biophysics

    Background:

    • Previous simulations focused on single nerve fibers using voltage clamp.
    • The double sucrose gap method is a common technique for voltage clamp experiments.

    Purpose of the Study:

    • To extend voltage clamp simulations to a bundle of axons.
    • To investigate the impact of series resistance and shunt pathways on voltage clamp accuracy in multicellular preparations.

    Main Methods:

    • Simulated a voltage clamp circuit applied to a unidimensional cable model representing a nerve fiber bundle.
    • Incorporated series resistance and a shunt pathway into the simulation model.
    • Analyzed the effects of voltage command steps on transmembrane potential and recorded current.

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    Main Results:

    • Series resistance greater than 50 omega-cm2 allowed propagated action potentials despite attempted voltage clamp.
    • Recorded currents did not exhibit 'notches' even with propagated action potentials.
    • Shunt pathways led to inaccurate current density measurements.

    Conclusions:

    • In multicellular preparations with series resistance, a recorded square voltage pulse does not guarantee voltage control of transmembrane potential.
    • Neither series nor shunt resistance accurately replicate the expected 'notches' in current records during voltage clamp.
    • The presence of series resistance significantly impacts the fidelity of voltage clamp experiments in nerve fiber bundles.