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A new type of allantoinase in amphibian liver
W Masuda1, S Fujiwara, T Noguchi
1Department of Biochemistry, Kyushu Dental College, Kokura, Kitakyushu, Japan. wmasuda@kyu-dent.ac.jp
Bioscience, Biotechnology, and Biochemistry
|January 17, 2002
Abstract:
Allantoinase and allantoicase are known to form a complex in amphibian liver. In this study, a new type of allantoinase that did not form a complex with allantoicase was found in the amphibian liver. Purified enzyme had a molecular mass of about 44 kDa both in SDS-PAGE and gel-filtrations. The enzyme cross-reacted with anti-sardine allantoinase polyclonal antibody, and it weakly cross-reacted with anti-bullfrog allantoinase polyclonal antibody.

