[Sensitivity of different cell cultures to 1-aminoadamantan]

Studies were carried out on the sensitivity of primary cell cultures of pig kidney (PK), pig thyroid gland (PTG), and chick embryo fibroblasts (CEF) to the synthetic viral inhibitor 1-amino adamantane (1-AA). The 1-amino adamantane was shown to have no effect on the cell growth in the cultures of PK and PTG in a concentration of up to 50 micrograms per cu. cm, and the CEF culture in a concentration of up to 25 mcg/cu. cm. When the cultures were treated after the cellular monolayer had been formed a concentration of 50 mcg/cu. cm initiated no cytotoxic effects in PK and CEF cultures in the course of 6-days while in PTG there were no such effects for twelve days. The cytotoxic changes consisted in the appearance of rounded and swollen cells, vacuolization of the cytoplasm, and deformation of the nuclei.