Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Angular dependent light scattering from multicellular spheroids.

J R Mourant1, T M Johnson, V Doddi

  • 1Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico 87545, USA. jmourant@lanl.gov

Journal of Biomedical Optics
|January 31, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observation of kinetic mix enhancement in thin-shell OMEGA implosions.

Physical review. E·2025
Same author

Revealing the Structure and Dynamics of Self-Generated Electric and Magnetic Fields Near Plasma Stagnation in Laser-Driven Hohlraums.

Physical review letters·2025
Same author

Design of an in-vacuum manipulator for nuclear diagnostics development at the MIT linear electrostatic ion accelerator.

The Review of scientific instruments·2025
Same author

Impact of x rays on the sensitivity of CR-39 detectors to 2.4-MeV protons.

The Review of scientific instruments·2025
Same author

Biermann-Battery-Driven Magnetized Collisionless Shock Precursors in Laser-Produced Plasmas.

Physical review letters·2025
Same author

Monte Carlo toolkit for designing and validating step-range-filter spectrometer designs.

The Review of scientific instruments·2025
Same journal

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026
Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026
Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
See all related articles

Light scattering is a promising tool for cancer detection. Cell shape and contact minimally impact light scattering, but internal cell structure and cell cycle differences are measurable, aiding diagnostics.

Area of Science:

  • Biophysics
  • Cell Biology
  • Medical Diagnostics

Background:

  • Light scattering is explored as a noninvasive method for tissue diagnostics, particularly cancer detection.
  • Understanding factors influencing light scattering from cells is crucial for developing accurate diagnostic tools.

Purpose of the Study:

  • To investigate the impact of cell-cell contact and cell shape on light scattering from mammalian fibroblast cells.
  • To determine if light scattering can differentiate between tumorigenic and nontumorigenic cells in a 3D culture system.

Main Methods:

  • Analysis of angular distribution of light scattering from mammalian fibroblast cells.
  • Comparison of light scattering properties between tumorigenic and nontumorigenic cells in a three-dimensional culture system.

Related Experiment Videos

Main Results:

  • Cell-cell contact and cell shape changes have a minor effect on light scattering angular distribution.
  • Significant differences in light scattering were observed between tumorigenic and nontumorigenic cells.
  • Differences are attributed to cell cycle variations: nontumorigenic cells arrest in G1, while tumorigenic cells proliferate.

Conclusions:

  • Internal cellular structure changes are measurable via light scattering, independent of cell organization.
  • Light scattering shows potential for distinguishing cell types based on proliferation status and internal structure.
  • This research supports the use of light scattering for noninvasive cancer detection by focusing on internal cellular changes.