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Related Experiment Videos

Anisomorphic cell division by African trypanosomes.

K M Tyler1, K R Matthews, K Gull

  • 1Northwestern University Medical School, Department of Pathology, Chicago, IL 60611, USA. k-tyler@northwestern.edu

Protist
|February 2, 2002
PubMed
Summary

African trypanosomes change shape in the bloodstream. Researchers used flagellar length to track cell division and differentiation, revealing distinct morphological changes during this process.

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Area of Science:

  • Cell Biology
  • Parasitology
  • Molecular Biology

Background:

  • African trypanosomes exhibit pleomorphism in mammalian hosts, transitioning between proliferative slender and non-proliferative stumpy forms.
  • Differentiation from slender to stumpy forms involves morphological intermediates and coordination with cell cycle exit.

Purpose of the Study:

  • To investigate the coordination between morphological change and cell cycle exit during African trypanosome differentiation.
  • To characterize slender form cell division and identify temporal markers for studying differentiation.

Main Methods:

  • Characterized slender form cell division in African trypanosomes.
  • Utilized new flagellum outgrowth as a temporal marker for the cell cycle.
  • Employed flagellar length as a marker for slender to stumpy differentiation.

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Main Results:

  • New flagellum outgrowth occurs at a linear rate, enabling precise temporal ordering of organelle segregation (nucleus, kinetoplast, mitochondrion).
  • Flagellar length effectively marks the transition from slender to stumpy forms.
  • Differentiating cells are anisomorphic, differing in flagellar length, cell shape/size, and nuclear morphology compared to proliferative cells.

Conclusions:

  • Flagellar length serves as a reliable marker for both cell cycle progression and differentiation in African trypanosomes.
  • The study provides insights into the coordinated regulation of cell division and differentiation in these parasites.
  • Morphological differences observed in differentiating cells highlight distinct cell cycle exit strategies.