Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Conservation of Protein Domains02:26

Conservation of Protein Domains

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Preliminary application of linear stapler-assisted en bloc resection of lateral pelvic sidewall for locally advanced rectal cancer and locally recurrent rectal cancer with significant pelvic occupation].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2026
Same author

[Analysis of the prevalence status and clinical characteristics of the hepatitis D virus in the Xinjiang region].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2025
Same author

[Development and reliability and validity testing of the questionnaire on rotavirus vaccination behavioral and social drivers].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025
Same author

[Clinical application of pelvic floor <i>en bloc</i> resection in combined pelvic organ resection for locally advanced or locally recurrent rectal cancer].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2025
Same author

[Reexamining the authorship of <i>Wen Yi Ming Bian</i>].

Zhonghua yi shi za zhi (Beijing, China : 1980)·2025
Same author

[Analysis of the application value of <sup>18</sup>F-FDG PET-CT in differentiating physiological uptake in the endometrium from stage IA endometrial carcinoma].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2025

Related Experiment Video

Updated: Jul 22, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

Computationally focusing the directed evolution of proteins.

C A Voigt1, S L Mayo, F H Arnold

  • 1Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, California 91125, USA.

Journal of Cellular Biochemistry. Supplement
|February 14, 2002
PubMed
Summary

Directed evolution enhances enzyme properties using computational methods. These strategies focus mutant library optimization, residue targeting, and recombination experiment design for improved enzyme function.

More Related Videos

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Related Experiment Videos

Last Updated: Jul 22, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Area of Science:

  • Biochemistry and Molecular Biology
  • Protein Engineering
  • Computational Biology

Background:

  • Directed evolution is a powerful strategy for modifying enzyme properties.
  • Traditional methods involve random mutations or gene crossovers.
  • Advancements in computational power enable more sophisticated approaches.

Purpose of the Study:

  • To describe computational methods for optimizing directed evolution.
  • To highlight techniques for targeted mutagenesis and recombination design.
  • To guide the development of improved enzyme variants.

Main Methods:

  • Computational algorithms for focused combinatorial searches.
  • Methods for optimizing mutant libraries.
  • Strategies for identifying specific residues for mutagenesis.
  • Design of recombination experiments using computational guidance.

Main Results:

  • Emergence of computational tools to guide enzyme evolution.
  • Facilitation of targeted approaches over random screening.
  • Potential for more efficient and effective enzyme modification.

Conclusions:

  • Computational methods are transforming directed evolution strategies.
  • These tools enable precise optimization of enzyme properties.
  • Future enzyme engineering efforts will benefit from computational guidance.