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Updated: Jul 22, 2026

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
Published on: November 7, 2012
Computationally focusing the directed evolution of proteins
C A Voigt1, S L Mayo, F H Arnold
1Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, California 91125, USA.
Abstract:
Directed evolution has proven to be a successful strategy for the modification of enzyme properties. To date, the preferred experimental procedure has been to apply mutations or crossovers randomly throughout the gene. With the emergence of powerful computational methods, it has become possible to develop focused combinatorial searches, guided by computer algorithms. Here, we describe several computational methods that have emerged to aid the optimization of mutant libraries, the targeting of specific residues for mutagenesis, and the design of recombination experiments.
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